Spatiotemporal evolution of microbial-induced calcium carbonate precipitation based on microfluidics

HE Xiang; LIU Han-long; HAN Fei; MA Guo-liang; ZHAO Chang; CHU Jian; XIAO Yang

doi:10.11779/CJGE202110012

Volume 43 Issue 10

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Chinese Journal of Geotechnical Engineering > 2021 > 43(10): 1861-1869. > DOI: 10.11779/CJGE202110012

HE Xiang, LIU Han-long, HAN Fei, MA Guo-liang, ZHAO Chang, CHU Jian, XIAO Yang. Spatiotemporal evolution of microbial-induced calcium carbonate precipitation based on microfluidics[J]. Chinese Journal of Geotechnical Engineering, 2021, 43(10): 1861-1869. DOI: 10.11779/CJGE202110012

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Spatiotemporal evolution of microbial-induced calcium carbonate precipitation based on microfluidics

HE Xiang^{1, 2,},
LIU Han-long^{1, 2, 3},
HAN Fei⁴,
MA Guo-liang^{1, 2},
ZHAO Chang^{1, 2},
CHU Jian⁵,
XIAO Yang^{1, 2, 3, ,}

1.
School of Civil Engineering, Chongqing University, Chongqing 400045, China
2.
Key Laboratory of New Technology for Construction of Cities in Mountain Area, Chongqing University, Chongqing 400045, China
3.
National Joint Engineering Research Center of Geohazards Prevention in the Reservoir Areas (Chongqing), Chongqing 400045, China
4.
University of New Hampshire, Hampshire 03824, USA
5.
School of Civil and Environmental Engineering, Nanyang Technological University, Singapore 639798, Singapore

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Received Date: December 24, 2020
Available Online: December 02, 2022

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Abstract

Abstract

The microbial-induced calcium carbonate precipitation (MICP) is a hot research topic in recent years, however, the understanding of its spatiotemporal evolution is still insufficient. This paper aims to investigate the spatiotemporal evolution process by designing a conceptual microfluidic chip within large and small pores and using a visualized experimental platform. An image-processing method is proposed to distinguish the calcium carbonate and measure its areas during the precipitation process, which allows the quantitative study on the spatiotemporal evolution process of MICP. The results show that the pore structures are involved in regulation of crystallization of the calcium carbonate. The calcium carbonate in the large pores of the channel exhibit as a single crystal, while those in the small pores between the sand particles show asymptotic growth in the form of aggregates and exhibit three different growth processes. Regardless of single crystal or crystalline aggregates, the growth rate of the calcium carbonate first increases and then gradually decreases with the increasing reaction time. The maximum growth rate is 4.22 μm/min with respect of the equivalent radius of the calcium carbonate crystals. This study is expected to benchmark the pore-scale modeling of biomineralization and provide reference for field practices.
- biomineralization,
- microfluidics,
- calcium carbonate,
- growth rate,
- MICP

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Spatiotemporal evolution of microbial-induced calcium carbonate precipitation based on microfluidics

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