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Volume 42 Issue 4
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WU Lin-yu, MIAO Lin-chang, SUN Xiao-hao, CHEN Run-fa, WANG Cheng-cheng. Experimental study on sand solidification using plant-derived urease-induced calcium carbonate precipitation[J]. Chinese Journal of Geotechnical Engineering, 2020, 42(4): 714-720. DOI: 10.11779/CJGE202004014
Citation: WU Lin-yu, MIAO Lin-chang, SUN Xiao-hao, CHEN Run-fa, WANG Cheng-cheng. Experimental study on sand solidification using plant-derived urease-induced calcium carbonate precipitation[J]. Chinese Journal of Geotechnical Engineering, 2020, 42(4): 714-720. DOI: 10.11779/CJGE202004014
shu

Experimental study on sand solidification using plant-derived urease-induced calcium carbonate precipitation

  • 1.

    Institute of Geotechnical Engineering, Southeast University, Nanjing 210096, China

  • 2.

    Shanghai Municipal Engineering Design Institute (Group) Co., Ltd., Shanghai 200092, China

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  • Received Date: May 20, 2019
  • Available Online: December 07, 2022
    Graphical Abstract
    • Abstract
  • The plant-derived urease-induced calcium carbonate precipitation cemented sand is a new technology in the field of geotechnical engineering, which has many advantages over the currently widely-used technology of solidifying sand by microorganism. Urease is extracted from soybeans directly. At first, the effects of temperature and pH on soybean urease activity are studied. Then the tests on the urease-induced calcium carbonate precipitation are carried out by controlling gel solution concentration, pH, temperature and reaction time. Based on this, three kinds of sand with different particle sizes are solidified by injecting urease solution and gel solution circularly. The solidification effect is evaluated by ultrasonic tests, unconfined compressive strength tests and calcium carbonate production tests. The results show that the optimal pH of soybean urease is 8, and urease activity increases with the increase of temperature in the range of 15℃~75℃. The precipitation product induced by soybean urease is calcite. With the increase of gel solution concentration, the production rate of calcium carbonate increases first and then decreases, and when the gel solution concentration is 0.75 mol/L, the production rate of calcium carbonate is the highest. When the gel concentration is constant, the production rate of calcium carbonate is the highest at the pH of 8, and the production rate increases with the increase of reaction time. The temperature within 10℃~40℃ has little effect on the production rate of calcium carbonate. The compressive strength of solidified samples is positively correlated with the content of calcium carbonate. With the increase of the particle sizes of sand, the compressive strength of samples increases first and then decreases, and the sand with particle size of 0.25~0.5 mm has the best solidification effect.
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    • References (21)
  • [1]
    DEJONG J T, MORTENSEN B M, MARTINEZ B C, et al. Bio-mediated soil improvement[J]. Ecological Engineering, 2010, 36(2): 197-210. doi: 10.1016/j.ecoleng.2008.12.029
    [2]
    VAN Wijngaarden W K, VERMOLEN F J, VAN Meurs G A M, et al. Modelling biogrout: a new ground improvement method based on microbial-induced carbonate precipitation[J]. Transport in Porous Media, 2011, 87(2): 397-420. doi: 10.1007/s11242-010-9691-8
    [3]
    孙潇昊, 缪林昌, 童天志, 等. 微生物沉积碳酸钙固化砂土试验研究[J]. 岩土力学, 2017, 38(11): 3225-3230. https://www.cnki.com.cn/Article/CJFDTOTAL-YTLX201711019.htm

    SUN Xiao-hao, MIAO Lin-chang, TONG Tian-zhi, et al. Experimental study of solidifying sand using microbial- induced calcium carbonate precipitation[J]. Rock and Soil Mechanics, 2017, 38(11): 3225-3230. (in Chinese) https://www.cnki.com.cn/Article/CJFDTOTAL-YTLX201711019.htm
    [4]
    DHAMI N K, REDDY M S, MUKHERJEE A. Biomineralization of calcium carbonates and their engineered applications: a review[J]. Frontiers in Microbiology, 2013, 4: 1-13.
    [5]
    CHU J, IVANOV V, HE J, et al. Development of microbial geotechnology in Singapore[C]//Geo-Frontiers 2011: Advances in Geotechnical Engineering, 2011, Dallas: 4070-4078.
    [6]
    DEJONG J T, FRITZGES M B, NÜSSLEIN K. Microbially induced cementation to control sand response to undrained shear[J]. Journal of Geotechnical and Geoenvironmental Engineering, 2006, 132(11): 1381-1392. doi: 10.1061/(ASCE)1090-0241(2006)132:11(1381)
    [7]
    HAMED Khodadadi T, KAVAZANJIAN E, VAN Paassen L, et al. Bio-grout materials: a review[C]//Grouting, 2017, Honolulu: 1-12.
    [8]
    DILRUKSHI R A N, WATANABE J, KAWASAKI S. Sand cementation test using plant-derived urease and calcium phosphate compound[J]. Materials Transactions, 2015, 56(9): 1565-1572. doi: 10.2320/matertrans.M-M2015818
    [9]
    HAMDAN N, KAVAZANJIAN E Jr, O'DONNELL S. Carbonate cementation via plant derived urease[C]//Proceedings of the 18th International Conference on Soil Mechanics and Geotechnical Engineering, 2013, Paris: 2-6.
    [10]
    KAVAZANJIAN E, HAMDAN N. Enzyme induced carbonate precipitation (EICP) columns for ground improvement[C]//IFCEE 2015, 2015, San Antonio: 2252-2261.
    [11]
    ALMAJED A A. Enzyme Induced Carbonate Precipitation (EICP) for Soil Improvement[D]. Phoenix: Arizona State University, 2017.
    [12]
    NEUPANE D, YASUHARA H, KINOSHITA N, et al. Applicability of enzymatic calcium carbonate precipitation as a soil-strengthening technique[J]. Journal of Geotechnical and Geoenvironmental Engineering, 2013, 139(12): 2201-2211. doi: 10.1061/(ASCE)GT.1943-5606.0000959
    [13]
    CARMONA J P S F, VENDA Oliveira P J, LEMOS L J L, et al. Improvement of a sandy soil by enzymatic calcium carbonate precipitation[J]. Proceedings of the Institution of Civil Engineers-Geotechnical Engineering, 2017, 171(1): 3-15.
    [14]
    LARSEN J, POULSEN M, LUNDGAARD T, et al. Plugging of fractures in chalk reservoirs by enzyme-induced calcium carbonate precipitation[J]. SPE Production & Operations, 2008, 23(4): 478-483.
    [15]
    NAM I H, CHON C M, JUNG K Y, et al. Calcite precipitation by ureolytic plant (Canavalia ensiformis) extracts as effective biomaterials[J]. KSCE Journal of Civil Engineering, 2015, 19(6): 1620-1625. doi: 10.1007/s12205-014-0558-3
    [16]
    JAVADI N, KHODADADI H, HAMDAN N, et al. EICP treatment of soil by using urease enzyme extracted from watermelon seeds[J]. Geotechnical Special Publication, 2018, 2018(GSP 296): 115-124.
    [17]
    WHIFFIN V S. Microbial CaCO3 Precipitation for the Production of Biocement[D]. Perth: Murdoch University, 2004.
    [18]
    SUN X H, MIAO L C, TONG T Z, et al. Study of the effect of temperature on microbially induced carbonate precipitation[J]. Acta Geotechnica, 2019, 14(3): 627-638. doi: 10.1007/s11440-018-0758-y
    [19]
    SUMNER J B. The isolation and crystallization of the enzyme urease preliminary paper[J]. Journal of Biological Chemistry, 1926, 69(2): 435-441.
    [20]
    HAMDAN N, KAVAZANJIAN E Jr. Enzyme-induced carbonate mineral precipitation for fugitive dust control[J]. Géotechnique, 2016, 66(7): 546-555.
    [21]
    DILRUKSHI R A N, KAWASAKI S. Plant-derived urease induced sand cementation used in geotechnical engineering applications[C]//International Conference on Geomechanics, 2016, Melbourne: 28-29.
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